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Quantitative real-time PCR Total RNA was isolated with Trizol from tumor as well as normal tissue samples collected from each patient and then purified using the RNeasy Mini Kit (Qiagen) according to the manufacturers protocol

doi:10.1017/s0007114511004703 Matsumoto M, Kitada Y, Naito Y

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Princeton, NJ, USA: 1999

However, in PD, dopaminergic neurons face overwhelming DNA damage due to oxidative toxicity from dopamine metabolites (e.g., dopamine quinones, H 2 O 2 ) and mitochondrial complex I deficiency-induced ROS bursts (OH), surpassing their repair capacity [65]

Many sources of prebiotics exist in nature including cereals, fruits and vegetables