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Materials and Methods Ethics Statement This study was carried out in strict accordance with the guidelines for the care and use of animals of Northwest A&F University

Colony formation assay We seeded PIK3CA -MT HCT116 colorectal cancer cells at a density of 2,000 cells per well in 6-well plates and allowed them to grow for 24 h

But if youre running multiple copper-containing compounds or high-dose protocols, total Cu 2+ exposure needs to be tracked

DO NOT spray the water directly onto the lyophilized powder cake

Serum IGF-1 levels, which would confirm that the blend is successfully stimulating the GH axis as expected

Use once or twice a day for best results