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Calcidiol can also be hydroxylated at the 24 position by a specific vitamin D 3 -24-hydroxylase (encoded by the CYP24A1 gene) in the kidneys, intestine, placenta, and cartilage which are all tissues that express the VDR

The liver injury pattern helps guide further testing such as ruling out primary biliary cholangitis or sclerosing cholangitis in cases of cholestasis or evaluating for biliary tract obstructions if jaundice is present [59]