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Reference Fc-GLP1 and G49 peptides were spiked into a 0.2% final concentration of nave mouse plasma in assay buffer (pH 7.4 HBSS supplemented with 25 mM HEPES, 0.1% BSA, and 0.5 mM 3-isobutyl-1-methylxanthine (IBMX)) at a known concentration to generate a standard curve to determine the amount of active peptide in each related plasma test sample