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Twenty four hours or a week after the last BrdU injection, rats were deeply anesthetized and transcardially perfused using PBS supplemented with 4 U ml 1 heparin (Merck Biosciences, Darmstadt, Germany), followed by fresh 2.5 % paraformaldehyde (J.T

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Jacobs A, Schutte AE, Ricci C, Pieters M

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This is a somewhat reducing environment that tends to keep protein cysteine residues in the thiol form (H PS SH ) and destabilizes any disulfide bonds they may make (P S S ), although very stable disulfide bonds found in some proteins could be present under these conditions