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Standard or sample was transferred in triplicate to a 96-well plate and GSH was then derivitized by the addition of 10 mM 2,3-naphthalenedicarboxyaldehyde and the fluorescence at Ex/Em wavelength of 472/528 nm was quantified using a fluorescent plate reader (Synergy 2, BioTek)

The disulfides can be cleaved by reducing agents such as beta-mercaptoethanol, dithiothreitol, or tris(2-carboxyethyl)phosphine (TCEP), or by oxidizing agents such as performic acid, which further oxidizes the disulfide to separate cysteic acids

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In general, interfering with SCL7A11 expression modulates System X c activity during ferroptosis, thereby regulating cancer cell replication, tissue invasion, and metastasis