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Without the copper, the peptide loses most of its biological activity

Antioxidant, antigenotoxic, and hepatic ameliorative effects of quercetin/zinc complex on cadmium-induced hepatotoxicity and alterations in hepatic tissue structure

J Biol Chem, 1998

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18 Lipofectamine 3000 Invitrogen L3000-015 Milk powder AppliChem A0830 Mowiol Sigma D2522 3-(4,5-Dimethylthiazol-2-yl)-2,5- diphenyltetrazoliumbromid (MTT) Sigma M2128 N-acetylcysteine (NAC) Sigma A9165 0.9% NaCl Braun 2350710 Nicotinamide adenine dinucleotide phosphate (NADPH) Biomol 16156 Nonidet-P40 (NP40) AppliChem 9016-45-9 DL-propargylglycine (PAG) Sigma P7888 PD184532 Axon Medchem 1368 Paraformaldehyde (PFA) Sigma P6148 PLX4032 Axon Medchem 1624 Puromycin Calbiochem 32438 5-Sulfosalicylic acid hydrate (SSA) Sigma 390275 SYBRGreen Invitrogen S7563 TEMED Roth 2367.3 TANGO buffer, 10x Thermo Fisher BY5 Taurine Sigma T0625 Triton-X100 Roth 3051.2 XtremeGene siRNA transfection reagent Roche 04476093001 XtremeGene9 DNA transfection reagent Roche XTG9-RO Xylavet (20mg/ml) Cp-pharma ' 4!!!!Material!and!Methods