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The HPLC chromatograms in Figure 3 AC indicate that there was no peak around the NAC and Di-NAC retention time in any of the experimental media: (i) placebo without NAC and Di-NAC, (ii) Mobile Phase and (iii) Milli-Q water

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This reduction in cortisol metabolism may increase fetal exposure to cortisol (202) and alter the programming of the hypothalamic-pituitary-adrenal axis, impacting neuroendocrine development (202, 203)