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PubChem Reference: View compound profile on NIH PubChem Key Characteristics MOLECULAR PROFILE Formula: C 78 H 123 N 23 O 23 S 2 Weight: 1815.12 g/mol CAS: 221231-10-3 Origin: hGH 177-191 + N-terminal tyrosine Structure: Modified peptide with disulfide bridge PHYSICAL PROPERTIES Form: White to off-white lyophilized powder Solubility: Readily soluble in water Stability: Excellent with disulfide bridge Storage: Keep refrigerated 3646 F (28 C) Related products NAD+ $40.00 $125.00Price range: $40.00 through $125.00 Ipamorelin $55.00 $95.00Price range: $55.00 through $95.00

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With 564 amino acid sequences (supplementary file rewriteEverything.out ) of semaglutide backbone in place, a new set of in silico approaches were employed for high-throughput structural modeling (Modeller [44]) and and K d calculations (Prodigy [45, 46]), which led to the identfication of a structural electrostatic scaffold at the binding interface of GLP-1R ECD and semaglutide analogue A747 (supplementary file A747.pdb ) with four site-specific missense mutations L23B R, I20B Q, V24B R and R25B A

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131 , S1S4 (1997)

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