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Indeed, the levels of several tight junction proteins, measured by Western blots, including occludin and ZO-1 were decreased in hypertensive models in some studies [92, 94], but not others, where differences in colonic occludin or ZO-1 between hypertensive and normotensive animals was not detected when these were assessed by immunofluorescence [93]

If side effects consistently appear 24-48 hours after injection (near peak concentration) and resolve by day five or six (as levels decline toward trough), you have strong evidence that your symptoms are dose-dependent rather than caused by some external factor