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Oxidation of disulfide bonds of Pt GSTF8 Disulfide bonds between C13 of purified apo- Pt GSTF8 variants and GSH were formed by incubating 20 M Pt GSTF8 with 0 mM or 1 mM GSSG in the presence of 200 M GSH in 10 mM MOPSO, pH 6.45 for 2 h at 30 C, 800 rpm using a 3 mm shaking diameter
ture Reviews Neuroscience9, 665-677 (September 2008)
While previous studies have investigated various OS markers in ECT-treated patients with schizophrenia [27, 30], the present study is the first to specifically examine GSSG levels and GSH/GSSG ratio changes following ECT in a Chinese schizophrenia population
PLoS ONE 9 , e103061 (2014)
The PVDF membranes were incubated with primary antibodies overnight at 4 C, incubated with secondary antibodies at room temperature for 1 h, and visualized using the Bio-Rad ChemiDoc XRS + system
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