Lo MY, Kim HT
The liver is responsible for filtering toxins, heavy metals, alcohol, and medications from the bloodstream, and glutathione acts as a shield binding to these harmful substances and facilitating their elimination
After deparaffinization, rehydrating, antigen retrieval, quenching and blocking, slides were incubated with primary antibody (-SMA, GFP (HuaBio, Cat# ET1602-7)) at 4 C overnight, applied with peroxidase conjugated secondary antibody in the diluent for 1 h at room temperature, stained with 3, 3-diaminobenzidine reaction solution, and imaged by Olympus digital slicing scanner VS200
The goal is to break open the cells and release their intracellular contents into the lysis buffer
[DOI] [PMC free article] [PubMed] [Google Scholar] (840).Runguphan W
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