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(2022) demonstrated in Nutrients that EVOO consumption reduced gut permeability (measured via zonulin) and metabolic endotoxaemia in patients with impaired fasting glucose, while simultaneously increasing postprandial GLP-1 and insulin levels

Cultures were pulse-labeled with 10 M BrdU (a nucleoside analog that competes with thymidine for incorporation into DNA) in DMEM-10% HS for the final 2-h period of 48-h in culture, followed by immunocytochemistry for detection of BrdU using G3G4 anti-BrdU mAb (1:100 dilution) and HRP-conjugated anti-mouse IgG Ab (1:500 dilution)

Rats in both experiments were euthanized in a CO 2 chamber at the end of the experiment

Am J Clin Pathol 154(Suppl 1):S29

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