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Low maternal plasma B 12 and high folate during gestation predict increased HPA-axis stress in the offspring

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For immunofluorescence staining, cells were rinsed three times with PBS before being incubated with BODIPY TM 581/591 C11 dye in serum-free medium at 37 C with 5% CO2 for 30 min before being visualized under a fluorescence microscope (Evos FL, Thermo Fischer Scientific) with objective 40

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(115) reported that microbiota-derived metabolites suppress HIF-2, a master regulator of intestinal iron absorption, and increase ferritin levels, thereby limiting host iron uptake and potentially reducing the labile iron pool that fuels lipid peroxidation

Additionally, they reduce oxidative stress, which may slow aging and enhance overall cellular health