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10.7554/eLife.02523 31 Do VanB.GouelF.JonneauxA.TimmermanK.GelP.PtraultM.et al (2016)

Proc Am Assoc Clin Oncol, 2004

1999 Aug;4(4):239248

The entire procedure typically takes under 1520 minutes, which allows people to quickly incorporate it into their hectic lifestyle

were produced using 5ug/mL of (13)--D-glucan from heat killed Candida albicans for 24 hours, as previously described ( in vitro experiments were stimulated with 5g/mL of (13)-B-D-glucan from Alcaligenes faecalis (Sigma-Aldrich), 100ng/mL of lipopolysaccharide (LPS) from Escherichia coli O127:B8 (Sigma-Aldrich), 120M L-buthionine sulfoximine (Sigma-Aldrich), and/or 1mg/mL of reduced glutathione (Sigma-Aldrich) for 24 hours unless otherwise stated in figure legends

Before You Begin: What You Will Need Having everything assembled before starting minimizes the time your lyophilized peptide is exposed to open air and reduces the risk of procedural errors