Western Blotting Purified protein samples were suspended in 1 sodium dodecyl sulfate (SDS) loading buffer (250 mM Tris-HCl pH 6.8, 40% glycerol (v/v), 10% SDS (w/v) (SB0485, Biobasic), 0.05% bromophenol blue (w/v) (0449-25G, Amresco), 5% 2-mercaptoethanol (M6250-100ML, Sigma)) and heated for 5 min at 95C
Ethics statement This study was reviewed and approved by The Institutional Animal Care and Use Committee at Nagasaki University
*Correspondence: Christian M
It is prepared in stringent conditions and contains no contaminants found in the consumer products of lower quality
Cell Cycle 17 , 903916 (2018)
Concurrently, the nanoreactors also activate the systemic immune system in mice, preventing tumor metastasis and recurrence