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Negative-staining electron microscopy Purified GLP-2RG s complex was diluted to 0.012 mg/mL with 20 mM HEPES, pH 7.4, 100 mM NaCl, 5 M GLP-2, 100 M TCEP, 2 mM MgCl 2 , 0.00075% (w/v) LMNG, 0.00025% (w/v) GDN and 0.0002% (w/v) CHS

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Binvignat, M., Sellam, J., Berenbaum, F

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avoid vigorous shaking Do NOT freeze after reconstitution Inspect solution before use: should be clear, colorless, and free of particulates Discard if cloudy, discolored, or precipitated Use sterile techniques during reconstitution and experimental handling Protect from heat, light, and mechanical agitation to preserve peptide integrity Retatrutide is most stable as a lyophilized powder stored refrigerated or frozen

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