Swisher SG , Roth JA, Lawrence D, Kemp B, Carrasco C, Fossella F, Glisson B, Hong WK, Khurie F, Kurie J, Nesbitt J, Pisters K, Putnam JB, Schrump D, Shin D, Walsh G
10.1016/j.bbrc.2021.05.011 143 SuL.-J.ZhangJ.-H.GomezH.MuruganR.HongX.XuD.et al (20192019)
Then, the membranes were incubated at 4 C overnight with primary antibodies against PKM1 (1:1000, Cell Signaling Technology, 7067), PKM2 (1:1000, Cell Signaling Technology, 4053), Nrf2 (1:1000, Cell Signaling Technology, 12721), -arrestin1 (1:1000, Cell Signaling Technology, 12697), -arrestin2 (1:1000, Cell Signaling Technology, 3857), 3-tubulin (1:1000, Cell Signaling Technology, 4466), -actin (1:1000, Cell Signaling Technology, 4970), Gclc (1:1000, Proteintech, 12601-1-AP), Gclm (1:1000, Proteintech, 14241-1-AP), and GFAP (1:1000, Millipore, MAB360)
[DOI] [PubMed] [Google Scholar] 24.Hamelers IH, et al
It is a very heavy, achy pain but it really dose hurt
Cold Preparation: Dilute the powder in 30ml of cold water