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The lysate was clarified by centrifugation at 15,000 nx g for 15 min, protein concentrations were determined using the BCA total protein assay (Pierce), and then appropriate volumes of the lysates were diluted with 2x reducing Laemmli buffer and heated for ten min at 95 C

Results in Figure 3A,B revealed a similar pattern of PCNA induction in tendon fibroblasts by BPC 157 and growth hormone that was correlated very well with the increase of viable cell number

However, they appear to contain large quantities of vitamin B12 analogues

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