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Here's where most protocols fail: never inject air into the peptide vial to 'equalise pressure'

Glutathione affinity purification, like many other types of affinity purifications, involves three key steps: 1) binding the target protein to the beads, 2) washing away contaminating proteins, and 3) eluting your protein of interest (Figure 3)

rSmGST has the typical C- and N-terminal double domains of glutathione transferase

J Cancer (2021) 12(9):2598609