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Competitive binding assay To investigate the competitive relationship among E3, Pelo, and Pns11, a competitive binding assay was performed as previously described 38

The Lancet, 392 (10156), 31-41

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Dissociated hippocampal neurons were treated with Hinge (10 " ' 2 M), HGF, Dihexa (10 "12 M) or HGF (lOng/ml) for 5 days after at which time mEPSCs were recorded in the absence of action potentials