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As mentioned earlier, this is crucial for accurate dosing

Fasting is required for rescuing hippocampal-dependent long-term memory in both sexes We examined the contribution of fasting on CR-induced improvements in cognition by performing behavioral assays on 12-month-old 3xTg and NTg mice that had been fed either an AL, DL, or CR diet prior to sacrificing them for the histological assays described above

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Adrian TE, Gariballa S, Parekh KA, Thomas SA, Saadi H, Al Kaabi J, et al