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When the expression of CRH in the SCN was examined using in situ hybridization, younger 3-4-month-old Sprague-Dawley rats exposed to light from 04.00h to 18.00h have a clear diurnal rhythm with higher expression seen in samples taken at 03.00h versus 23.00h

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The condensation reaction can be carried out at room temperature, sometimes requiring reflux at a certain temperature

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