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doi: 10.2337/db22-1406-P 93 OlsenMBHovelmannUGriffinJKnudsenKMJohansenTKendallDet al

In vitro studies have confirmed that cellular SIRT1, SIRT2 [the most highly expressed sirtuin in the brain (Supplementary Table 1)], SIRT3, SIRT5, and SIRT6 are regulated by [NAD + ] and not by the [NAD + ]/[NADH] (Anderson et al., 2017), as non-physiological millimolar levels of NADH were required to inhibit sirtuin activity in vitro (Madsen et al., 2016)

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