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Stromal vascular fraction isolation For stromal vascular fraction (SVF) isolation, harvested sWAT depots from male C57Bl/6J mice were finely minced and digested in collagenase solution (2 mg/ml collagenase Type II, 10 mM CaCl2 and 0.5% free fatty acid free BSA in PBS) for 30 min at 37 C at 16 g

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[DOI] [PMC free article] [PubMed] [Google Scholar] 14.Miyagawa T, Kawamura H, Obuchi M, Ikesaki A, Ozaki A, Tokunaga K, et al

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However, further studies are needed to fully understand their effects, particularly on EAT and HFpEF [43]

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