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FastQC_v0.115 tool ( was used separately to analyze both paired-end sequencing read files, and necessary trimming was achieved by FASTX Toolkit v0.0.14 ( The estimation of the best suitable kmer size in the processed reads of the genome of strain MBM was made by employing kmergenie_v1.7051 software with auto Kmer mode as the default parameter [66]

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Step 1: Gather Your Supplies Your lab bench should be clean and organized