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Tubulin expression was detected on the same PVDF membrane using anti--tubulin specific monoclonal antibody (1 g/ml Sigma-Aldrich, Ont., CA), followed by HRP-conjugated goat anti-mouse IgG (1:5000 (v/v), BioRad, Ont., CA)

However, compounded medications are not FDA-approved as finished products and have not undergone FDA review for safety, efficacy, or manufacturing quality in their final form

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Caffeine-based lipolysis and cAMP stimulation release fat from cells without disrupting the cell itself the fat can be re-stored if not fully metabolized