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Studies demonstrate FOXO4-DRI achieves comparable renal function restoration to genetic clearance of senescent cells using the p16-3MR suicide gene system, confirming that therapeutic effects result specifically from senescent cell elimination rather than off-target actions

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ROS drive HIF-1-BNIP3/NIX-mediated mitophagy and alleviate cell apoptosis and inflammation in M9a+sh EPAS1 cells during hypoxia To identify the cause of induced mitophagy, we explored the role of ROS, given their involvement in stabilizing HIF-1 [22, 28]

Studies typically employ subtype-selective antagonists and receptor-expression controls to deconvolute specific receptor contributions to measured biochemical endpoints across cell-based assays and in-vivo systems