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All the reagents should be mixed thoroughly by gently swirling before pipetting

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1DF, half-maximal GEP44 binding at GLP-1R was observed at 113 nM ( versus 5.85 nM for Ex-4), 65.8 nM at Y2-R ( versus 1.51 nM for PYY 3-36 ), and 86.6 nM at Y1-R ( versus 7.9 nM for PYY 1-36 )

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