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The extraction mixture content was 90% methanol and an internal standard for Kynurenine and tryptophan (Sigma-Aldrich, St

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Individuals with B12 deficiency, which can occur due to dietary restrictions (e.g., vegan or vegetarian diets), gastrointestinal disorders affecting absorption, older age, or certain medical conditions that interfere with B12 absorption

7D), versus MSG control group

Many contain hydrophobic regions that readily bind to plastic surfaces, pulling active peptide out of solution and depositing it on the container walls