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Immunostaining revealed mixed cell populations with many undifferentiated hPSCs and mesoderm cells, and DE markers SOX17 and FOXA2 were expressed at lower levels compared to the Activin A-based control (CA)

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After incubation with TruStain fcX -mouse CD16/32 blocking antibody (BioLegend), staining of different surface markers (see Table 1) was performed for 20 min at 4 C followed by a washing step with PBS/1% FCS

Smoday I.M., Petrovic I., Kalogjera L., Vranes H., Zizek H., Krezic I., Gojkovic S., Skorak I., Hriberski K., Brizic I., et al

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