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The role of macula densa nitric oxide synthase 1 beta splice variant in modulating tubuloglomerular feedback

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The reaction incubated for 15 min and 6 l of 50 mM DTNB was added to each well

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RNA samples were hybridized at 45C overnight in 30 pl of 20 mM Tris-HCI, pH 7.5, containing 500 mM NaC1, I mM EDTA, and 75% deionized formamide with 5.10' cpm of the "P- labeled antisense rihoprobe