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The protein oligomers were separated by 8% SDS-PAGE, transferred to 0.22 m PVDF membrane, and blocked with 5% non-fat milk in TBS-T (20 mM Tris-HCl pH 7.6, 150 mM NaCl, and 0.01% Tween 20)

You need to have an intrinsic factor, a protein made by cells in your gut lining to bring B12 into circulation, transcobalamin proteins to move it around the body and finally, pancreatic enzymes for absorption

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TB-500, its synthetic fragment, gained scientific credibility when Malinda et al