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Mistake 1: Shaking the vial This is the most common mistake and the most destructive

The restraints were derived from (i) NOE restraints from intermolecular and intramolecular cross-peaks in NOESY spectra (described in NMR spectroscopy section), (ii) TALOS derived p53 TAD2 and FOXO4-DRI backbone dihedral angles, (iii) hydrogen bond restraints (regions p53 residues 4752, FOXO4-DRI residues 918

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The microdose case is best framed as metabolic or maintenance translation, not proven 72-week obesity dosing