Using a Flexstation 3 plate reader, a baseline luminescence reading was acquired for 8 minutes at 37C, followed by stimulation with vehicle or 100 nM exendin-4 for Nb37 bystander assay, or with serial dilutions of exendin-4 starting from 1 M, 100 nM, 10 nM to 1 nM for 30 minutes for the mini-G s / mini-G q / -arrestin 2 recruitment assays
A helix propensity scale based on experimental studies of peptides and proteins
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(168) assessed the body fat composition, IR, leptin, and lipid profile in 55 individuals with PWS and 18 obese controls
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