RNA isolation and quantitative RT-PCR ESCC samples were flash frozen in liquid nitrogen and homogenized with a mortar and pestle, as previously described
Vidal-Alaball J, Butler CC, Cannings-John R, Goringe A, Hood K, McCaddon A, McDowell I, Papaioannou A
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Each batch is verified using HPLC, Mass Spectrometry, and UV analysis
25 MarinMMarcocciCVittiPChiovatoLBartalenaL
Although TET members such as TET2 functions as tumor suppressor in certain cancers including AML and glioma 45 , we showed that knockdown of individual TET members consistently abrogates the YBX1AMPK axis in liver and lung cancer cells, suggesting potential functional synergy among TET members in regulating local DNA demethylation, although the underlying mechanisms warrant further investigation