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Library preparation was followed by clean up and size selection using SPRI beads (Beckman Coulter Genomics) aiming for an insert size of 350 bp

This review examines the core molecular mechanisms of ferroptosis and cuproptosis and delineates their respective roles in OSCC initiation, progression, immune evasion, and therapeutic resistance

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The vector was transformed into CodonPlus-RIL cells and expressed in LB supplemented with 100 g/mL ampicillin and 35 g/mL chloramphenicol