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Moreover, ferroptosis in this cell line can be assessed using the classic cytotoxic lactate dehydrogenase (LDH) assay ( see figures )

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These effects are dose-dependent and typically peak during dose titration, consistent with receptor desensitization over several weeks

Available from: Mathew J, Sankar P, Varacallo M

Using this method Orci was able to chart the journey of insulin from its site of synthesis, as proinsulin, through to its release