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Sakuma S., Ishimura M., Yuba Y., Itoh Y., Fujimoto Y

On DIV12 the neurons were fixed (4% paraformaldehyde, 3% sucrose, 60 raM PIPES, 25 mM HEPES, 5 mM EGTA, 1 niM MgCl 2 , pH 7.4) for 20 min at room temperature and mounted

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because rigorously controlled human safety data specific to the TB-500 fragment does not exist, its longer-term risk profile, including possible immune reactions, remains unknown Evidence snapshot: Evidence for TB-500 itself is preclinical, drawn mainly from cell-culture and animal studies (a 1999 rat wound-healing study and later rodent cardiac- and tendon-injury models), with a 2026 scoping review describing the musculoskeletal literature on thymosin beta-4 and TB-500 as sparse and largely preclinical

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