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doi: 10.1016/j.ad.2023.06.017
Companion satellite-cell cultures, prepared at the same time, were immunostained at 30-h post-plating with D3 anti-desmin mAb and DAB (a chromogenic HRP-substrate that forms an insoluble brown reaction product) to determine the percentage of myogenic cells present 18 ,19 cultures with less than 95% DAB-positive cells (namely, less than 95% desmin-positive myogenic purity) were not used for experiments just to ensure the reliability of data specific to myogenic cells in the employed primary cell culture systems
Furthermore, glutathione synthesized from glutamine-derived precursors confers treatment resistance by scavenging therapy-induced reactive oxygen species, thereby suppressing radiation- and chemotherapy-triggered autophagic cell death and establishing a self-perpetuating oncogenic circuit (110, 111)
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Knowing the total quantity helps researchers plan and scale their experiments accurately
