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Typically, freezing medium in laboratory research consists of 10 vol% DMSO in Dulbeccos modified Eagles medium (DMEM) containing 20 vol% foetal bovine serum (FBS)

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Glucuronidation Increases BUT Is Insufficient While glucuronidation (the other safe pathway) does increase during pregnancy: This increase in glucuronidation CANNOT compensate for the simultaneous: 33% decrease in sulfation (another safe pathway) 80% increase in oxidation to toxic NAPQI **The Result:** When both safe pathways become saturated or overwhelmed, significantly more acetaminophen is forced through the toxic CYP2E1 pathway, creating 43% more NAPQIat precisely the time when glutathione reserves are most depleted

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Effect of iron limitation and fur gene inactivation on the transcriptional profile of the strict anaerobe Clostridium acetobutylicum