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Plasma glucose concentration was determined by an enzymatic colorimetric test

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This protocol aligns with natural circadian rhythms and metabolic patterns

it results in the loss of 60% of the fatty acids present in the plasma membrane, inversely affecting membrane fluidity, enhancing the permeability of ions, and inhibiting the actions of enzymes and receptors, as well as compromising sperm membrane integrity, defective motility, and reduced sperm-oocyte interaction [39]