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The Journal of general physiology.139(6)

Mice were sacrificed at 6, 12, and 24 h post-injection through anesthesia followed by exsanguination via open heart puncture

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In the context of AD, the inflammatory responses of M1 microglia, which are predominantly localized around the amyloid plaques, are initiated and sustained by P47 phagocyte oxidase and NF-B (nuclear factor kappa B) (130)

V.MikiT.et al (2011)