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On day 6, cells were counted, and 100 l cell suspension (in DMEM media supplemented with only 10% FBS but no antibiotics) was added to the 96-well plates (containing siRNA and transfection mix) at the appropriate seeding density (12k for DMSO and 15k for etoposide-treated cells)

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The nasal route theoretically offers direct nose-to-brain transport via the olfactory nerve pathway, bypassing the blood-brain barrier

Res Exp Med (Berl)

a , MichaelisMenten plot of measured reaction rate at different substrate concentration