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Apoptosis in human sperm: its correlation with semen quality and the presence of leukocytes

Antibodies against MARCO (Abcam, clone EPR22944-66, catalog ab259264, 1:200), F4/80 (Invitrogen, Thermo Fisher Scientific, clone CI:A3-1, catalog MA1-91124, 1:250), and CLEC4F (R&D Systems, Bio-Techne, catalog AF2784, 1:200) were incubated overnight at 4C

The lysates were sonicated, separated via centrifugation, and loaded onto a GSTrap FF column (17513001, GE Healthcare)

First, this study was limited to a single dose and fasting conditions because we excluded the effect of diet and examined the direct effect of GLP-1RA

It is used with a reduced-calorie eating plan and increased physical activity for chronic weight management in certain people with obesity or overweight with weight-related health conditions

There is no robust clinical evidence showing that semaglutide directly reduces cortisol levels, and it should not be used for this purpose