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Next, the protein was eluted using LDOX elution buffer (20 mM potassium phosphate, 200 mM NaCl, 2.5 mM d -desthiobiotin, pH 7.4) and the fractions were combined according to the recorded absorption 280 nm ( A 280nm )

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The above figure emphasizes another aspect of the proposed studies, the relationship between S and N excretion

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In addition, in the case-control part we carefully matched for sex, age as well as geographic region and used multivariable analysis to further adjust for potential confounders such as physical activity, levels of education and smoking status