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Physiological thinning of the teat canal keratin layer before parturition and transient dilation of the teat sphincter after milking are considered key windows for pathogen invasion 186

On the other hand, exclusion criteria included (1) review articles with poor experimental data, (2) research studies without clear methodologies, (3) research articles unrelated to pharmaceutical prospect of antioxidants and (4) publications without scope of our research work, (5) non-peer-reviewed studies, (6) literature older than 25 years, as well as (6) unpublished work

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doi: 10.3201/eid2303.161552 248 WilkinsonT

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The protein concentration in the samples was determined using Bradfords assay