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Human clinical trials remain sparse

The enzyme activity was measured after addition of 6 mM of NAD to the tissue supernatant mixture in a cuvette at 340 nm against a blank (without NAD)

doi: 10.1210/jc.2012-3888 119 GoldstoneAPHollandAJHauffaBPHokken-KoelegaACTauberM

Inhibition of glucagon secretion secondary to the increase in insulin secretion cannot plausibly explain the effects either, because a similar reduction in glucagon levels has also been reported in patients with type 1 diabetes [51]

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