Several considerations are typically accounted for, besides accurate mass and RT, which includes the elimination of background signals, deisotoping, identification of adduct peaks in MS spectra, and MS/MS fragmentation patterns compared to databases or internal standards and the matching degree estimated (see Figure 2)
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For Safranin O/Fast Green staining, slides were stained with hematoxylin (Mayer, Sigma) for 5 min, 0.005% Fast Green (Thermo Fisher Scientific, Pittsburgh, PA, United States) for 1 min, rinsed with 1% acetic acid, and stained with 0.1% Safranin O (Millipore, Billerica, MA, United States) for 20 min
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