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Cells were cultured in Dulbeccos modified Eagle medium (DMEM, Gibco) with 10% (v/v) fetal calf serum (FCS, Thermo Fisher) and Penicillin/Streptomycin (PenStrep) at 37 C with a 5% CO 2 atmosphere

Metabolite sensing mechanisms and switches (such as O-GlcNAcylation, N-acylation, lactylation and methylation) have been discovered to dynamically regulate the epigenetic programming of particular lineages cell fates during fetoplacental development